ENZYMATIC OXIDATION OF PYRIDOXAMINE PHOSPHATE TO PYRIDOXAL PHOSPHATE IN RABBIT LIVER
نویسندگان
چکیده
منابع مشابه
Enzymatic oxidation of pyridoxamine phosphate to pyridoxal phosphate in rabbit liver.
In the course of testing the possible coenzymic function of the phosphorylated vitamin B, derivatives on the synthesis of glucosamine 6-phosphate by a rat liver preparation, the appearance of a yellow color similar to that of pyridoxal 5-phosphate was observed in the samples containing pyridoxamine 5-phosphate. Further investigation has revealed the presence of a soluble enzyme system in extrac...
متن کاملPyridoxamine phosphate-oxidase and pyridoxal phosphate-phosphatase activities in Escherichia coli.
acetone), than found in earlier investigations. Mayer (1930) used a concentration of 66% for his determinations, but this is definitely inhibitory, though lese so for insoluble preparations than for soluble. The enzyme is remarkably stable before it becomes soluble and the activity of soluble preparations is retained well in the cold. The optimum temperature for enzyme action is much lower than...
متن کاملThe enzymatic oxidation of 1,2-propanediol phosphate to acetol phosphate.
Lindberg (I) has presented evidence for the occurrence of 1,2propanediol phosphate in beef brain. This compound has also been reported to occur in other tissues (2-4). However, the structure, metabolism, and biological significance of this compound is unknown. Groth et al. (5) have reported that (Y-labeled pyruvate is incorporated into propanediol-P’ under anaerobic conditions in fluoride-inhib...
متن کاملThe enzymatic oxidation of pyridoxine and pyridoxamine phosphates.
Extracts of rabbit liver and brain are known (1, 2) to oxidize pyridoxine 5-phosphate to pyridoxal 5-phosphate much faster than they oxidize free pyridoxine to pyridoxal. Comparison of the two activities during limited purification indicated that different enzymes catalyzed the two reactions (1). Pogell (3) had shown previously that oxidation of pyridoxamine 5-phosphate was catalyzed by an enzy...
متن کاملHomogeneous, nonradioactive, enzymatic assay for plasma pyridoxal 5-phosphate.
BACKGROUND Pyridoxal 5'-phosphate (PLP) is the biologically active form of vitamin B(6). Clinical studies suggest that low PLP concentrations are an independent risk factor for cardiovascular and other diseases. However, PLP concentrations are not routinely diagnosed because of the lack of a homogeneous, nonradioactive assay. We describe a homogeneous, nonradioactive, enzymatic PLP assay that u...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1958
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)77396-7